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Concurrent knockdown of p53 specifically ameliorates the cell death induced by MO off-targeting.. p53 activation has also recently been shown to be an unspecified off-target effect of siRNAs.
One of the notable and unexpected findings from our work using MOs has been a series of observations surrounding unanticipated effects that are independent of the intended gene target.
p53 activation by knockdown technologies
p53 activation by knockdown technologies Type With Light On Logitech 039;s New Solar Keyboard For Mac
Knockdown of spliceosomal proteins and treatment with Life Technologies ScienceOpen: research and publishing network.. Vertebrate knockdown strategies—such as morpholinos (MOs) in zebrafish or RNA interference-based strategies in mammalian systems—have been demonstrated to be effective, rapid, and cost-efficient reverse-genetic approaches for studying gene function. Media Center For Mac
The major off-targeting effect is mediated through p53 activation, as detected through the transferase-mediated dUTP nick end labeling assay, acridine orange, and p21 transcriptional activation assays.. However, their deployment has to date been limited by a number of technical (genomic, biological, and off-targeting) hurdles.. We have identified and characterized a recently described p53 induction pathway due to off-targeting that appears to be shared between knockdown technologies.. Importantly, reversal of p53-dependent cell death by p53 knockdown does not affect specific loss of gene function, such as the cell death caused by loss of function of chordin.. p53 inhibition could potentially be applicable to other systems to suppress off-target effects caused by other knockdown technologies. sd mmc navione navione exe
Both technologies, however, can elicit undesirable off-target effects We have used several model genes to study these effects in detail in the zebrafish, Danio rerio.. This indicates that DNA damage is not responsible for the observed activation of p53.. To top Page menu Author and article information; Abstract p53 Activation by Knockdown TechnologiesRecent advances in sequence-based approaches to “knockdown” gene function have opened the door to an array of approaches to uncover functions for genes of interest.. Using the zebrafish embryo as a template, correct and mistargeting effects are readily discernible through direct comparison of MO-injected animals with well-studied mutants.. We show here indistinguishable off-targeting effects for both maternal and zygotic mRNAs and for both translational and splice-site targeting MOs.. Both commonly used knockdown technologies can thus induce secondary but sequence-specific p53 activation. b0d43de27c Gratis Video Youtube Idm Tidak Muncul
